产品说明
一般描述
Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.
应用
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.
Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
包装
25, 100 g in poly bottle
特点和优势
- Excellent recovery
- Minimal sample dilution
- Quick and easy to use
法律信息
Sephadex is a registered trademark of Cytiva
基本信息
| MDL编号 | MFCD00132771 |
产品性质
| 质量水平 | 200 |
| 等级 | DNA grade for molecular biology |
| 产品线 | BioReagent |
| 形式 | beads |
| 溶胀 | 1 g swells to 9-11 mL |
| 珠子直径 | 50-150 μm |
| 孔径 | exclusion limit (20 bp dsDNA; (rA)20) |
| pH值(酸碱度) | 2-10 |
| 异质活性 | DNase, RNase, none detected |
| 储存温度 | room temp |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
