Milli-Mark^® Anti-TrkA-FITC Antibody

产品说明

一般描述

TrkA is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.

特异性

Unconjugated version Cat.No.06-574 is predicted to react with Rat based on immunogen design. The unconjugated part number was also shown to react with Mouse.
Antibody recognizes TrkA protein.

免疫原

Bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
Epitope: Extracellular domain

应用

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases
Research Category
Neuroscience
Detect TrkA using this Milli-Mark Anti-TrkA-FITC Antibody validated for use in FC.

质量

Evaluated by flow cytometry using HeLa cells.

目标描述

TrkA exists as two isoforms: 87 kDa and 88 kDa

外形

Purified rabbit polyclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Protein A purified

储存及稳定性

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
HeLa cells

法律信息

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质

安全信息