产品说明
一般描述
In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
特异性
Antibody recognizes TRA-2-49.
免疫原
2102Ep human Embryonal Carcinoma Cells
Epitope: Unknown
应用
| Working Solution | Cells / test | Total Reaction Volume |
|---|---|---|
| 5 μl | 5 x 105 Cells / 95 μl PBS | 100 μl |
| 10 μl | 1 x 106 Cells / 90 μl PBS | 100 μl |
Research Sub Category
Pluripotent & Early Differentiation
Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Research Category
Stem Cell Research
质量
Evaluated by flow cytometry using 2102 Ep cells.
目标描述
212/80/55 kDa Observed
外形
Protein A purified
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
储存及稳定性
Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
分析说明
Control
2102 Ep cells.
法律信息
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 偶联物 | FITC conjugate |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | TRA-2-49/6E, monoclonal |
| species reactivity | human |
| manufacturer/tradename | Milli-Mark® |
| technique(s) | flow cytometry: suitable |
| 同位素/亚型 | IgG1 |
| 运输 | wet ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
