Lipase A Candida antarctica, recombinant from Aspergillus oryzae

产品说明

一般描述

Lipase A Candida antarctica (CALA) belongs to the α/β hydrolase family. The catalytic triad comprises Ser184, His366, and Asp334 residues.

应用

Lipase A Candida antarctica, recombinant from Aspergillus oryzae has been used in the enzyme-catalyzed hydrolysis studies with 3-acetylthiohexanal. It has also been used as a biocatalyst to test its effect on the esterification of phytosterol with caprylic acid.

生化/生理作用

Lipase A Candida antarctica (CALA) is a thermostable serine hydrolase with a higher sn-2 preference towards triglycerides. It displays excellent activity towards large substrates as well as bulky sterically hindered tertiary alcohols.
Lipase A from Candida Antarctica catalyzes hydrolysis of tertiary alcohols to form glycerol and fatty acids and shows selectivity for the N-acylation of β-amino esters.

单位定义

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40℃ (triolein, Cat. No. 62314, as substrate); 1 U as described above is equivalent to ~0.15 U using trybutyrine, Cat. No. 91010, as substrate, at pH 8.0 and 70℃

其他说明

Characterization

基本信息

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