产品说明
一般描述
Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
细胞系来源
Skin
应用
injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response
组分
Fibroblast Basal Medium containing 10% FBS & 10% DMSO
制备说明
- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
传代培养常规
Please refer to the HDF Culture Protocol.
基本信息
| NACRES | NA.81 |
产品性质
| 质量水平 | 100 |
| 生物来源 | human neonatal foreskin or adult skin (normal) |
| 包装 | pkg of 500,000 cells |
| 生长模式 | Adherent |
| 核型 | 2n = 46 |
| 形态学 | Fibroblast |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
安全信息
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
