产品说明
一般描述
FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.
应用
Fas Ligand (FASLG) human has been used for-
- the study of FASLG stimulation in luteal cells obtained from mid-corpus luteum (CL) and
- the study of apoptosis induction and intracellular caspase-3 assay in human cells.
生化/生理作用
Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).
FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E. In CD4+ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.
其他说明
Human Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acids 134-281.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.
分析说明
Measured by its ability to induce apoptosis in Jurkat cells.
基本信息
| MDL编号 | MFCD01092558 |
| NACRES | NA.32 |
产品性质
| 质量水平 | 100 |
| 重组 | expressed in CHO cells |
| 测定 | >95% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 分子量 | monomer calculated mol wt ~18 kDa 26-28 kDa by SDS-PAGE |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P48023 |
| 储存温度 | −20℃ |
| Gene Information | human ... FASLG(356) |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H315 - H319 |
| 预防措施声明 | P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Skin Irrit. 2 |
| 储存分类代码 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
