产品说明
一般描述
2× 浓缩的即用型热启动预混液，用于 qPCR 和 qRT-PCR，使用实时 PCR 仪器上的水解探针检测格式。
EagleTaq Universal Master Mix (ROX) 是一种即用型 2× 浓缩 PCR Master Mix，包含了进行定量、实时 PCR 水解探针反应所需的所有试剂(引物、探针和模板除外)。它包含一种特殊的 ROX 引用染料，使其适用于所有需要使用 ROX 引用染料进行定量分析的实时仪器。
EagleTaq Universal Master Mix (ROX) 含有 dUTP，因此可与尿嘧啶-DNA 糖基化酶 (UNG) 配合使用，以防止携带污染引起的假阳性。
热启动属性允许在环境温度下设置反应。
有了这种耐用的试剂，任何 PCR 方案优化都能最小化
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.
应用
EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR).
特点和优势
- The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
- It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
- The hot start properties allow reaction setup at ambient temperature.
- With this robust reagent, any PCR protocol optimization is minimized.
组分
含有 EagleTaq DNA 聚合酶和 dATP、dCTP、dGTP、dUTP、MgCl 2 和 ROX 的反应缓冲液。
产品性质
| 质量水平 | 100 |
| 用途 | sufficient for 100 reactions sufficient for 500 reactions sufficient for 5000 reactions |
| 特点 | dNTPs included hotstart |
| manufacturer/tradename | Roche |
| 包装 | pkg of 100 x 20 μL reactions (07260288190) pkg of 500 x 20 μL reactions (07260296190) pkg of 5000 x 20 μL reactions (07249926190) |
| technique(s) | RT-qPCR: suitable qPCR: suitable |
| input | purified DNA |
| detection method | probe-based |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | does not flash |
| 闪点(C) | does not flash |
