产品说明
一般描述
FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.
应用
Fas Ligand (FASLG) from mouse has been used for-
- the induction of apoptosis in PC12 cells and
- the induction of migration in BV-2 murine microglial cells.
生化/生理作用
Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).
FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E. In CD4+ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.
其他说明
Mouse Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acid residues 132-279.
外形
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
分析说明
Measured by its ability to induce apoptosis in Jurkat cells.
基本信息
| MDL编号 | MFCD00285792 |
| NACRES | NA.32 |
产品性质
| 重组 | expressed in mouse NSO cells |
| 质量水平 | 100 |
| 测定 | >95% (SDS-PAGE) |
| 形式 | lyophilized powder |
| 分子量 | monomer calculated mol wt ~18 kDa 28-32 kDa by SDS-PAGE |
| 杂质 | endotoxin, tested |
| UniProt登记号 | P41047 |
| 储存温度 | −20℃ |
| Gene Information | mouse ... Fasl(14103) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
