产品说明
一般描述
EIF2AK2 (eukaryotic translation initiation factor 2-α kinase 2) is a ubiquitously expressed mammalian Ser/Thr kinase. It is localized to cytoplasm as well as nucleus. Its substrate is α subunit of eukaryotic initiation factor 2 (eIF2α), and it is a constitutively expressed enzyme. This protein is divided into two functional regions- its N-terminal contains two dsRNA binding domains (dsRBDs) and the C-terminal has the kinase domain. In dormant cells, it remains inactivated, but under proper stimuli it either self-activates or is activated by PKR activating protein.
生化/生理作用
EIF2AK2 (also known as double-stranded RNA-activated protein kinase) is a protein kinase that has been shown to be involved in HIV/gp120-associated neurodegeneration.EIF2AK2 acts as a critical mediator of gp120 neurotoxicity and is a substrate for a family of protein kinases that respond to various forms of environmental stress. Activation of EIF2AK2 leads to its autophosphorylation and then phosphorylation of its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2. EIF2AK2 plays a critical role in mRNA translation, cell proliferation and apoptosis. A novel cross-talk between the EIF2AKs and p53 has been shown that has implications in cell proliferation and tumorigenesis.
外形
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
法律信息
PRECISIO is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.32 |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in baculovirus infected Sf9 cells |
| 产品线 | PRECISIO® Kinase |
| 测定 | ≥70% (SDS-PAGE) |
| 形式 | buffered aqueous glycerol solution |
| specific activity | 51-68 nmol/min·mg |
| 分子量 | ~64 kDa |
| UniProt登记号 | P19525 |
| 运输 | dry ice |
| 储存温度 | −70℃ |
| Gene Information | human ... EIF2AK2(5610) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
