产品说明
免疫原
synthetic peptide corresponding to amino acids 151-170 of human doublecortin, conjugated to KLH. The corresponding sequence is identical in rat and mouse doublecortin.
应用
Anti-Doublecortin antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2μg/mL using HEK-293T cells expressing human doublecortin, and mouse brain and rat brain extract (S1 fraction).
Anti-Doublecortin antibody produced in rabbit has been used in immunoblotting and immunohistofluorescence.
生化/生理作用
Doublecortin is a 40kDa microtubule-associated protein (MAP) that is essential for migration of neurons during the development of the cerebral cortex. Mutations in the human DCX gene cause lissencephaly (smooth brain) or subcortical laminar heterotopia (SCLH), characterized by mental retardation and seizures. DCX interaction with MAPs stabilizes microtubules (MTs). The interaction with MTs is via a conserved N-terminal doublecortin (DC) domain. DCX can be regulated by phosphorylation at multiple sites by several kinases, including JNK, Cdk5, PKA, and MARK/PAR-1 family of protein kinases.
外形
Solution in 0.01 M phophate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD03454961 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~40 kDa |
| species reactivity | mouse, human, rat |
| 增强验证 | recombinant expression Learn more about Antibody Enhanced Validation |
| 浓度 | ~1 mg/mL |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cells expressing human doublecortin. Also, mouse and rat brain extract (S1 fraction). |
| UniProt登记号 | O43602 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... DCX(1641) mouse ... Dcx(13193) rat ... Dcx(84394) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
