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Anti-DHFR, N-terminal antibody produced in rabbit

品牌
Sigma-Aldrich
货号
D1067
规格纯度
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
参考价格
5045.46 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Dihydrofolate reductase has 187 amino acids and corresponds to molecular weight of 18-20 kDa. It is mapped to chromosome 5q22 region. DHFR contains substrate binding site and coenzyme binding domain.

特异性

Anti-DHFR, N-terminal antibody is specific for the epitope residing within amino acids of mouse DHFR. The antibody interacts with DHFR and DHFR fusion proteins. The product is specific for DHFR in humans, mice and rats. Staining of the DHFR band by immunoblotting is inhibited by the immunizing peptide.

免疫原

The immunizing sequence is conserved in mouse, human and rat.

应用

Anti-DHFR, N-terminal antibody produced in rabbit has been used in immunoprecipitation and western blotting.

生化/生理作用

Dihydrofolate reductase (DHFR) inhibitors, such as methotrexate, are folate analogs which can bind to the active site and deactivate the enzyme. From another angle, structural and enzymatic properties of DHFR led to development of a variety of screenings, in which DHFR can function in a fashion that resembles reporter genes. The screenings are based on the fact that DHFR can be dissected into two halves that can reassemble to form an active enzyme. Thus, each of the two halves of DHFR can be expressed as two fusion proteins that when interacting with each other can restore the DHFR enzymatic activity. The readout of this protein-protein interaction and consequent enzymatic activity, can be either restoration of growth in bacterial, yeast and plant, or receptor activation. The approach was proved to be useful for receptor-ligand, antigen-antibody and other interactions.
Dihydrofolate reductase (DHFR) is an NADPH dependent enzyme that reduces dihydrofolate and regenerates tetrahydrofolate. DHFR is involved in the biosynthesis of thymidylate and purines, and thereby regulates DNA synthesis and cell survival. Alterations in DHFR have been associated with impaired nucleic acid synthesis and cell death. Thus, this enzyme has important therapeutic implications in cell proliferative disorders such as cancer.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD05665212
NACRESNA.41

产品性质

生物来源rabbit
质量水平200
偶联物unconjugated
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式buffered aqueous solution
species reactivitymouse, rat, human
浓度~1.0 mg/mL
technique(s)immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR
western blot (chemiluminescent): 0.5-1.0 μg/mL using 100 ng of purified recombinant DHFR
UniProt登记号P00374
运输dry ice
储存温度−20℃
Gene Informationhuman ... DHFR(1719)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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