产品说明
一般描述
Coelenterazine (CTZ), a small prosthetic group, is a luciferin or light-producing compound present in luminous marine organisms. The imidazopyrazinone ring in CTZ is responsible for its luminous properties. This chemiluminescent dye is soluble in methanol and poorly soluble in water. CTZ can be oxidized with the help of bovine serum albumin (BSA) and several other luciferases.
应用
Coelenterazine - CAS 122384-88-7 – Calbiochem may be used:
- to determine superoxide production
- to determine free radical production
- in cell suspension buffer for in vivo reconstitution of aequorin
包装
250 μg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Coelenterazine (CTZ) is a cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion. It can also be used for detecting changes in intracellular Ca2+ in cells that have been transfected with apoaequorin cDNA. CTZ functions as a powerful antioxidant. It serves as a substrate for multidrug resistance protein 1 (MDR1) P-glycoprotein (ATP-binding cassette sub-family B member 1 (ABCB1)/Pgp) and can bind with apoaequorin to form the active aequorin complex. It can be mainly used to quantify the formation of superoxide in neutrophils.
警告
Toxicity: Standard Handling (A)
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C26H21N3O3 |
| 分子量 | 423.46 |
| MDL编号 | MFCD00467176 |
产品性质
| 质量水平 | 100 |
| 测定 | ≥90% (TLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze protect from light |
| 颜色 | brown |
| 溶解性 | methanol: 1 mg/mL ethanol: soluble |
| 运输 | ambient |
| 储存温度 | −20℃ |
| InChI | 1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2 |
| InChI key | YHIPILPTUVMWQT-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
