产品说明
一般描述
Anti-Cy3/Cy5 antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma CY-96 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from Balb/c mice immunized with a mixture of proteins labeled with Cy3 or Cy5.
特异性
The antibody recognizes Cy3 and Cy5 conjugated to proteins.
免疫原
mixture of proteins labeled with Cy3/Cy5.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Anti-Cy3/Cy5 antibody, Mouse monoclonal has been used in:
- immunofluorescence
- western blot
- dot blot
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunocytochemistry
- protein microarrays
- In in situ hybridization
生化/生理作用
Cy 3 and Cy5 are the most popular cyanine dyes, used combined for two color detection. Cy3 dyes are fluorescent orange while Cy5 is fluorescent in the red region. Cyanine belonging to polymethine group. CyDyes are a family of fluorophores that can be used for labeling proteins, peptides, DNA, RNA, and other biomolecules. These dyes are small, pH insensitive, soluble in aqueous solution and are tolerant to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. Mainly Cy3 and Cy5 are used in many different biological assays such as DNA microarrays, protein microarrays, two-dimensional protein analysis (2D gels), fluorescence resonance energy transfer (FRET), and immunocytochemistry.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | CY-96, monoclonal |
| 形式 | buffered aqueous solution |
| 浓度 | ~1.5 mg/mL |
| technique(s) | direct ELISA: suitable dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5 immunocytochemistry: suitable immunoprecipitation (IP): suitable microarray: suitable |
| 同位素/亚型 | IgG2a |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
