产品说明
一般描述
Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.
特异性
The antibody recognizes Cy3 and Cy5 conjugated to proteins.
免疫原
mixture of proteins labeled with Cy3/Cy5.
应用
Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
- dot blot assay
- protein microarrays
- immunofluorescenc
Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.
生化/生理作用
Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.46 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | biotin conjugate |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | CY-96, monoclonal |
| 形式 | buffered aqueous solution |
| 浓度 | ~1 mg/mL |
| technique(s) | direct ELISA: suitable dot blot: suitable immunocytochemistry: 1-2 μg/mL using chicken fibroblasts microarray: suitable |
| 同位素/亚型 | IgG2a |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
