产品说明
一般描述
Monoclonal Anti-Cathepsin K (mouse IgG2b isotype) is derived from the hybridoma CK124-1H6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human cathepsin K.
Cathepsins are proteases that normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell. Cathepsins are classified based on their functions and presence of active site such as cathepsin B, D, K, V, F etc.
特异性
The antibody does not cross react with the closely related lysosomal cysteine proteases cathepsins L, B, H, S, V and W.
免疫原
recombinant human cathepsin K. The antibody epitope resides within the peptide sequence RGYREIPEGNEKAL that corresponds to amino acids 222-235.
应用
Monoclonal Anti-Cathepsin K antibody produced in mouse has been used in western blotting and enzyme linked immunosorbent assay (ELISA).
生化/生理作用
Cathepsin is involved in various functions such as intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Among all the types, cathepsins K (Ctsk) is highly expressed in osteoclasts that plays a crucial role in bone remodeling and resorption. Cathepsin K protein was found in primary breast carcinoma and prostate cancer where it performs in the invasive process of prostate cancers, thyroid carcinoma, lung adenocarcinoma and breast carcinoma. Its deficency causes pycnodysostosis, a rare bone dysplasia with an osteopetrotic-like phenotype.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD02097890 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | CL124-1H6, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~27 kDa |
| species reactivity | human |
| 浓度 | ~2 mg/mL |
| technique(s) | indirect ELISA: suitable western blot: 10-20 μg/mL using total cell extracts of HCT-116 cells |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | P43235 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... CTSK(1513) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
