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Monoclonal Anti-μ-Calpain (Domain III) antibody produced in mouse

品牌
Sigma-Aldrich
货号
C266
规格纯度
clone 9A4H8D3, ascites fluid, buffered aqueous solution
参考价格
7037.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Calpain-1 catalytic subunit is a protein encoded by the CAPN1 gene in humans. Calpains are calcium dependent proteases constituting a family of proteins. They share a homologous cysteine-protease domain and an E-F hand Ca2+-binding domain. The calpain system consists of two ubiquitous forms of calpain (m-calpain and μ-calpain), a tissue specific calpain (n-calpain) and a calpain inhibitory protein (calpastatin).

特异性

Epitope mapping studies indicate the epitope is between amino acids 465-520 (domain III) of human μ-calpain. The antibody reacts specifically with μ-calpain. It does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. It is not recommended for immunoprecipitation. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and with rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.

免疫原

μ-calpain from bovine skeletal muscle.

应用

Monoclonal Anti-μ-Calpain (Domain III) antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:100 and western blotting at a dilution of 1:2,000.

生化/生理作用

Calpain is involved in calmodulin-independent pathway for the activation of calcineurin. Endogenous calpain I forms active calcineurin in the human heart by proteolysis of calcineurin A, which may lead to pathogenesis of myocardial disease. μ-calpain, on overexpression, may have relationship with intractable epilepsy as well as the clinicopathological characteristics. The activity of this protein is elevated in skeletal muscle from gastric cancer patients.

外形

Solution in phosphate buffered saline and contains 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD01322347
NACRESNA.41

产品性质

质量水平200
生物来源mouse
偶联物unconjugated
抗体形式ascites fluid
antibody product typeprimary antibodies
克隆9A4H8D3, monoclonal
形式buffered aqueous solution
分子量antigen 80 kDa
species reactivityrat, pig, human, bovine
technique(s)indirect immunofluorescence: 1:100
western blot: 1:2,000
同位素/亚型IgG1
UniProt登记号P07384
运输dry ice
储存温度−20℃
Gene Informationhuman ... CAPN1(823)
rat ... Capn1(29153)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKnwg
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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