产品说明
一般描述
Bcl-X has two isoforms, Bcl-XL (long), a 241-aa protein, and Bcl-XS (short), a 178-aa protein lacking a 63 aa domain that is well conserved among members of the Bcl-2 family.
特异性
MAB4625 recognizes a protein of 27 kDa, identified as the Bcl-X protein. Reacts with both Bcl-XS and Bcl-XL.
免疫原
Synthetic peptide, aa 3-14 of human Bcl-X protein. This amino acid sequence is shared by human and murine Bcl-X protein.
应用
Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
Wstern Blot: 1μg/mL (Hsu, 1997a; Hsu, 1997b)
Flow Cytometry
Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).
Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.
Optimal working dilutions must be determined by end user.
This Anti-Bcl-X Antibody, clone 2H12 is validated for use in FC, IF, IP, WB, IH for the detection of Bcl-X.
外形
Protein A purified immunoglobulin in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Format: Purified
储存及稳定性
Maintain at 2-8°C for up to 12 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 2H12, monoclonal |
| species reactivity | rat, human, pig, mouse |
| manufacturer/tradename | Chemicon® |
| technique(s) | flow cytometry: suitable immunofluorescence: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG2a |
| NCBI登记号 | NM_001191.2 NM_138578.1 |
| UniProt登记号 | Q07817 |
| 运输 | wet ice |
| Gene Information | human ... BCL2L1(598) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
