位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Ezrin Antibody, clone 4A5, Ascites Free

品牌
Sigma-Aldrich
货号
MAB3822-C
规格纯度
clone 4A5, from mouse
参考价格
5520.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Ezrin (UniProt Q8HZQ5; also known as Cytovillin, Villin-2, p81) is encoded by the EZR (also known as VIL2) gene (Gene ID 100008846) in Oryctolagus cuniculus (Rabbit) species. Ezrin, radixin, and moesin are the three ERM protein family members that crosslink actin filaments with plasma membranes and play an important role in the cell cortex architecture. In its inactive state, ezrin is located in the cytosol in a conformation involving intramolecular interaction between its N- and C-terminal. Ezrin is activated by phosphorylation, which causes a conformation change that disrupts the head-to-tail intramolecular interaction. Upon activation, ezrin binds to the cytoplasmic tails of multiple adhesion and signaling membrane molecules, such as CD44, growth factor receptors, and vascular adhesion molecule (VCAM1). The initiator methionine of ezrin is removed posttranslationally to yield the mature protein (a.a. 2-586) with an N-termina FERM domain (a.a. 2-295) that mediates interaction with microtubules.

特异性

Clone 4A5 reacted with both dentured and non-denatured Ezrin (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).

免疫原

Purified Ezrin from rabbit gastric parietal cells.

应用

Research Category
Cell Structure
Anti-Ezrin Antibody, clone 4A5, Ascites Free is an antibody against Ezrin for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Electron Microscopy, Immunocytochemistry.
Western Blotting Analysis: 0.5 µg/mL of this antibody from a representative lot detected Ezrin in 10 µg of HeLa cell lysate.
Immunohistochemistry Analysis: A representative lot detected a higher Ezin immunoreactivity in paraffin-embedded human medulloblastoma sections when compared with normal cerebellum sections (Haeberle, H., et al. (2012). Neoplasia. 14(7):666-669).
Immunohistochemistry Analysis: A representative lot immunostained parietal cells in rabbit gastric mucosa (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Ezin from human A431 cells. The immunoprecipitated Ezrin was phosphorylated on Ser66 from dbcAMP‐treated, but not cAMP‐treated, A431 cells (Wang, S., et al. (2009). Cell Res. 19(12):1350-1362).
Electron Microscopy Analysis: A representative lot detected Ezrin on microvilli of the intracellular canaliculi (IC) and the subapical region of resting parietal cells in Lowicryl HM20-embedded rabbit gastric gland ultrathin sections (Sawaguchi, A., et al. (2004). J. Histochem. Cytochem. 52(1): 77–86).
Immunocytochemistry Analysis: A representative lot detected exogenously expressed human GST fusion protein by fluorescent immunocytochemistry staining of formaldehyde-fixed, Triton X-100-permeabilized rabbit gastric parietal cells (Zhou, R., et al. (2003). J. Biol. Chem. 278(37):35651-35659).
Western Blotting Analysis: A representative lot detected both the endogenous rabbit Ezrin as well as exogenously expressed human Ezrin GST fusion proteins in transfected rabbit gastric parietal cells (Zhou, R., et al. (2003). J. Biol. Chem. 278(37):35651-35659).
ELISA Analysis: Clone 4A5 hybridoma culture supernatant was screened for its immunoreactivity by ELISA and found to react with both dentured and non-denatured 80 kDa Ezrin preparations from rabbit gastric glands/mucosa vesicular fractions (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).
Research Sub Category
Adhesion (CAMs)

质量

Evaluated by Western Blotting in L6 rat myoblast lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ezrin in 10 µg of L6 rat myoblast lysate.

目标描述

~75 kDa observed. 69.41 kDa (Human/UniProt P15311 & Mouse/P26040), 69.39 kDa (Rat/UniProt P31977), and 69.22 kDa (Rabbit/Q8HZQ5) calculated. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

质量水平100
生物来源mouse
抗体形式purified immunoglobulin
antibody product typeprimary antibodies
克隆4A5, monoclonal
species reactivityrat, mouse, human, rabbit
technique(s)electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型IgG1κ
NCBI登记号NP_001075591
UniProt登记号Q8HZQ5
运输wet ice
Gene Informationhuman ... EZR(7430)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号