位置:首页 > 品牌 > Sigma-Aldrich >

Cas9 RFP Lentiviral Particles

品牌
Sigma-Aldrich
货号
CAS9RFPV
包装型号
规格纯度
参考价格
3752.73 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Ready-to-use Cas9-RFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-RFP lentiviral particles efficiently and stably integrate Cas9 and RFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed.

To order gRNA in any format click here

应用

Functional Genomics/Target Validation

  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Creation of cell lines stably expressing Cas9-RFP

特点和优势

  • Highly specific
  • Highly active
  • Ready to infect/transduce

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

单位定义

VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.

法律信息

CRISPR Label License

Lentiviral, WPRE, and Evrogen Licenses

产品性质

包装pkg of 8 x 25 μL (aliquots)
浓度≥5 x 10 8 VP/ml virus (via p24 assay)
application(s)CRISPR
报告基因RFP
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号