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Anti-trimethyl Histone H3 (Lys79) Antibody

品牌
Sigma-Aldrich
货号
07-952
规格纯度
from rabbit, purified by affinity chromatography
参考价格
6694.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.

特异性

This antibody recognizes Histone H3 methylated at Lys79.
Broad species reactivity is expected.

免疫原

Epitope: Lys79
Linear Peptide corresponding to human Histone H3 methylated at Lys79.

应用


Dot Blot Specificity Analysis:
AbSurance Histone H3 Antibody Specificity Arrays (Cat. #: 16-667 and 16-665) containing peptides to all key histone modification sites, were probed with Anti-trimethyl Histone H3 (Lys79) Antibody Antibody ; dilution 1:5000. Proteins were visualized using a goat anti-mouse IgG (H+L) conjugated to HRP and a chemiluminescence detection system.


Multiplex Immunoassay
Specificity of interaction of anti-trimethyl histone H3 Lys79 antibody was assayed using the Luminex multiplex immunoassay platform. Anti-trimethyl-Histone H3 (Lys79) diluted to 0.01 mg/ml concentration was incubated with a cocktail of xMAP microspheres conjugated to the following histone H3 peptides, monomethyl Lys79, unmodified Lys79, trimethyl Lys 27, trimethyl Lys 9, trimethyl Lys79 processed and detected using standard xMAP protocols.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology
Anti-trimethyl Histone H3 (Lys79) Antibody is a rabbit polyclonal antibody for detection of trimethyl Histone H3 (Lys79). It has been tested for use in Western blotting, Dot blots, and Multiplex assays.

质量

Evaluated by Western Blot in HeLa acid extract lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Histone H3 on 10 µg of HeLa acid extract lysate.

目标描述

~ 17 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa acid extract lysate and recombinant Histone H3

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman
technique(s)dot blot: suitable
multiplexing: suitable
western blot: suitable
NCBI登记号NP_003484.1
UniProt登记号Q16695
运输wet ice

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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