产品说明
一般描述
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a widely expressed substrate of PKC. MARCKS is essential in neuronal development, as mice deficient in it die shortly after birth. Through its basic effector region, MARCKS associates with acidic membrane phospholipids, actin filaments, and calmodulin. Poly (ADP-ribose) also binds the basic effector region, blocking calmodulin binding, membrane association, and phosphorylation by PKC, suggesting that MARCKS may be an effector of the DNA-damage signal transduced by poly (ADP-ribose).
特异性
MARCKS, phosphoSer152/156.
免疫原
Synthetic peptide corresponding to amino acids surrounding the phosphoSer152/156 of rat MARCKS.
Epitope: phosphoSer 152/156
应用
Research Sub Category
Cytoskeletal Signaling
Research Category
Signaling
Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of MARCKS With Citrate pH 6.0 Epitope Retrieval: Rat Placenta
Optimal working dilutions must be determined by the end user.
This Anti-phospho-MARCKS (Ser152/156) Antibody is validated for use in WB, IH(P) for the detection of phospho-MARCKS (Ser152/156).
质量
Routinely evaluated by Western Blot on EGF treated A431 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phosphorylated MARCKS on 10 μg of EGF treated A431 lysates.
目标描述
~80 kDa
联系
Replaces: AB5879
外形
Purified rabbit polyclonal IgG in 10 mM Hepes, pH 7.5, with 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Affinity purified
储存及稳定性
Stable at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析说明
Control
A431 Cell Lysate
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | rat, mouse, human |
technique(s) | immunohistochemistry: suitable (paraffin) western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_002356.5 |
UniProt登记号 | P29966 |
运输 | wet ice |
Gene Information | human ... MARCKS(4082) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |