产品说明
一般描述
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways.
特异性
Recognizes the dual serine phosphorylated Smad1 (Ser463/465).
免疫原
peptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein
应用
Detect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
质量
routinely evaluated by immunoblot on RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4
目标描述
65 kDa
外形
Protein A purified
Format: Purified
分析说明
Control
BMP-4-treated HeLa or NIH/3T3 cells
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
产品性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | Xenopus, rat, mouse, human |
manufacturer/tradename | Upstate® |
technique(s) | western blot: suitable |
同位素/亚型 | IgG |
NCBI登记号 | NM_005900 |
UniProt登记号 | Q15797 |
运输 | dry ice |
Gene Information | human ... SMAD1(4086) |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 1 |