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Anti-SETD8 (hPR-SET7) Antibody

品牌
Sigma-Aldrich
货号
06-1304
规格纯度
from rabbit, purified by affinity chromatography
参考价格
6694.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

N-lysine methyltransferase KMT5A (UniProt: Q9NQR1; also known as EC: 2.1.1.43, H4-K20-HMTase KMT5A, Histone-lysine N-methyltransferase KMT5A, Lysine N-methyltransferase 5A, Lysine-specific methylase 5A, PR/SET domain-containing protein 07, PR-Set7, PR/SET07, SET domain-containing protein 8) is encoded by the KMT5A (also known as PRSET7, SET07, SET8, SETD8) gene (Gene ID: 387893) in human. SETD8 (hPR-SET7) is a histone methyltransferase that adds a single methyl group to lysine 20 of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. SETD8 is not detected during the G1 phase. It is first detected during S through G2 phases, and peaks during mitosis. It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It is required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis and is also involved in chromosome condensation and proper cytokinesis. It is also reported to mediate mono-methylation of p53/TP53 at lysine 382, leading to repress p53/TP53-target genes. SETD8 has a SET domain in the amino acids 257-378 and this region contains the active site of enzymatic activity. Sequences upstream and downstream of SET domain are also required for its methyltransferase activity. Two isoforms of SETD8 have been described that are produced by alternative splicing.

特异性

This rabbit polyclonal antibody detects N-lysine methyltransferase KMT5A (PR-SET7).

免疫原

GST-tagged recombinant protein corresponding to human SETD8 (hPR-SET7).

应用

Research Sub Category
Histones
Anti-SETD8 (hPR-SET7) Antibody is a Rabbit Polyclonal Antibody for detection of SETD8 (hPR-SET7) also known as Histone-lysine N-methyltransferase SETD8 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected SETD8 (hPR-SET7) in HeLa cell nucleaar extract.

目标描述

~43 kDa

联系

Replaces: 07-316

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable at 2-8°C for 1 year from date of receipt.

分析说明

Control
HeLa nuclear extract.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman
species reactivity (predicted by homology)rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)western blot: suitable
NCBI登记号NP_065115.3
UniProt登记号Q9NQR1
运输wet ice
Gene Informationhuman ... SETD8(387893)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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