产品说明
一般描述
Galectins specifically bind to β-galactosides. 15 mammalian galectins have been identified so far. Galectin 8 contains two CRDs connected by a short linker peptide.
Monoclonal Anti-Galectin 8 (mouse IgM isotype) is derived from the hybridoma VA-11.25 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant rat galectin 8.Galectins belong to a family of carbohydrate-binding proteins. Within this family of proteins, galectin 8 is unique, as it exists in many forms encoded by the same gene.
特异性
The antibody has low cross-reactivity with galectin 3 and galectin 9.
免疫原
recombinant rat galectin 8.
应用
Monoclonal Anti-galectin-8 antibody produced in mouse has been used for:
- immunoblotting
- immunocytochemistry
- enzyme linked immunosorbent assay (ELISA)
生化/生理作用
Galectins belong to a family of carbohydrate-binding proteins. These proteins share similarities in the carbohydrate recognition domain (CRD) and in their specificity for N-Acetyllactosamine-enriched glycoconjugates. The binding of galectins to saccharides modulates cell proliferation, cell death and cell migration, which are involved in cancer initiation and progression. Galectin 8 is a secreted protein that requires both of its CRDs to be active to modulate cell adhesion. It forms a complex with integrins and triggers integrin-mediated signaling cascades. On the contrary, excessive amounts of this protein negatively regulate cell adhesion by interacting with integrins. These signals assist in identifying regions available for cell adhesion and migration. It may be involved in neoplastic transformation.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | VA-11.25, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~34 kDa |
| species reactivity | rat, human, mouse |
| 浓度 | ~1.0 mg/mL |
| technique(s) | immunocytochemistry: suitable indirect ELISA: suitable western blot: 1-2 μg/mL using rat galectin 8 recombinant protein |
| 同位素/亚型 | IgM |
| UniProt登记号 | O00214 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... LGALS8(3964) mouse ... Lgals8(56048) rat ... Lgals8(116641) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
