Anti-WD repeat-containing protein 5 Antibody

产品说明

一般描述

WD repeat-containing protein 5 (WDR5) or alternatively BMP2-induced 3kb gene protein, is encoded by the human gene named WDR5/BIG3 and is part of a family of WD-40 repeat proteins that appear to facilitate the formation of multi-protein complexes much like a sticky tape will bind a variety of components together. The WD repeat proteins are involved in a wide array of cellular functions and processes including cell cycle progression, homeobox gene activation, apoptosis, gene regulation and histone methylation. One of WDR5 best studied activities is as part of a multi-protein complex that facilitates the methylation of histones, particularly histone H3, through its interaction with MLL a histone methyltransferase. WDR5 is also expressed in developing bone cells including osteoblasts, chondrocytes, and marrow stromal cells and works to accelerate osteoblast differentiation and positively promotes Wnt signaling in developing bone tissues.

免疫原

Linear peptide corresponding to human WD repeat containing protein 5.

应用

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Western Blotting Analysis: A representative lot from an independent laboratory detected WD repeat containing protein 5 in tested cell lysates. (Wysocka, J., et al. (2003). Genes Dev. 17(7):896-911.; Tyagi, S., et al. (2007). 27(1):107-119.; Jin, S., et al. (2010). J Clin Invest. 120(2):593-606.; Tyagi, S., et al. (2009). EMBO J. 28(20):3185-3195.; Diao, Y., et al. (2012). Cell Stem Cell. 11(2):231-241.; Schram, A. W., et al. (2013). PLoS One. 8(7):e70035.).
This Anti-WD repeat-containing protein 5 antibody is validated for use in western blotting for the detection of WD repeat-containing protein 5.

质量

Evaluated by Western Blotting in NIH/3T3 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WD repeat containing protein 5 in 10 µg of NIH/3T3 cell lysate.

目标描述

~37 kDa observed

外形

Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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