产品说明

一般描述

TREX1, (DNase III), or three prime repair exonuclease 1 is the major 3’→ 5’ DNA exonuclease in mammalian cells. TREX1 binds preferentially to ssDNA and can also remove mismatched 3’ terminal deoxyribonucleotides at DNA strand breaks. The protein is required for proper DNA checkpoint signaling and plays critical roles in DNA replication and gap filling during DNA repair. TREX1 has a perinuclear distribution in mammalian cells and is colocalized with ER-associated proteins in cells at rest. TREX1 relocalizes to the nucleus and specifically to replication forks during S phase and periods of DNA synthesis and repair such as after irradiation. TREX1 acts on an ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates and thus acts to attenuate DNA damage checkpoint signaling and prevent pathological immune activation in normal cells. Defects in TREX1 protein have been associated in humans with many inherited diseases including Aicardi-Goutiėres syndrome type 1 (AGS1), systemic lupus erythematosus (SLE), chilblain lupus type 1 (CHBL1), and vasculopathy, retinal, with cerebral leukodystrophy (RVCL). EMD Millipore’s Anti-TREX1 rabbit polyclonal antibody has been tested in western blotting, immunohistochemistry, and immunofluorescence in human samples.

免疫原

KLH-conjugated linear peptide corresponding to human TREX1.

应用

Detect DNASE using this rabbit polyclonal antibody, Anti-TREX1 Antibody validated for use in western blotting, IHC & Immunofluorescence.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected TREX1 in human spleen tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected TREX1 in human spleen tissue.

质量

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TREX1 in 15 µg of human spleen tissue lysate.

目标描述

~30 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 3 isoforms at ~32 kDa, ~33 kDa, and ~39 kDa

外形

Antigen Affinity Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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