Anti-NRF2a/GABPA Antibody

产品说明

一般描述

GA-binding protein alpha chain (UniProt Q06546; also known as GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60) is encoded by the GABPA (Also known as E4TF1A) gene (Gene ID 2551) in human. Initially identified through their interactions with cytochrome c and cytochrome oxidase promoters, GABPA (NRF-2) and the related nuclear respiratory factor NRF-1 target many nuclear genes essential for mitochondrial respiratory function, including the mitochondrial transcription specificity factors TFB1M and TFB2M. GABPA plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPA is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

免疫原

Recombinant protein corresponding to human NRF2a/GABPA.

应用

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected GABPA (NRF-2alpha) occupancy at the TFB1M and TFB2M promoters by ChIP using HeLa chromatin preparations (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Western Blotting Analysis: A representative lot detected GABPA (NRF-2alpha) in U2OS cell lysates (Gleyzer, N., and Scarpulla, R.C. (2011). J. Biol. Chem. 286(46):39715-39725; Gleyzer, N., and Scarpulla, R.C. (2013). J. Biol. Chem. 288(12):8004-8015).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using recombinant GABPA or heparin agarose-purified HeLa nuclear extract and radiolabeled synthetic oligonucleotides corresponding to cytochrome oxidase subunit IV, hTFB1M, or hTFB2M promoter sequence with GABPA recognition sites (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using in vitro translated GABPA and radiolabeled cytochrome oxidase subunit IV promoter fragment containing tandem GABPA recognition sites (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GABPA (NRF-2alpha)-PRC complex from human embryonic kidney 293FT cell lysate (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
This Anti-NRF2a/GABPA Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay for the detection of NRF2a/GABPA.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NRF2a/GABPA in 10 µg of HeLa cell lysate.

目标描述

~55 kDa observed

外形

Unpurified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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