产品说明
一般描述
PTPRF or leukocyte common antigen-related protein (LAR) is a widely expressed protein tyrosine phosphatase with an extracellular receptor region that resembles a cell adhesion molecule.
Protein tyrosine phosphatase, receptor type F (PTPRF) consists of noncovalently bound subunits designated as E (extracellular) and P (phosphatase) subunits that are derived from a precursor protein by proteolytic processing. The E subunit (150 kDa) has three immunoglobulin-like domains and eight fibronectin III-like domains, and the P subunit (85 kDa) has a short extracellular domain, a transmembrane segment and tandem cytoplasmic PTP catalytic domains.
特异性
The antibody recognizes both the extracellular and the cytoplasmic subunits of human PTPRF. Anti-PTPRF (C-terminal) antibody is specific for the P- subunit and the whole PTPRF protein from Human origin. Detection of the PTPRF bands by immunoblotting is specifically inhibited by the immunizing peptide.
应用
The antibody may be used in several immunochemical techniques including immunoblotting (∼210, ∼150, and ∼85 kDa) and immunoprecipitation.
Anti-PTPRF (C-terminal) antibody produced in rabbit has been used in an enzyme based assay of protein tyrosine phosphatase 1 (PTP1).
生化/生理作用
Protein tyrosine phosphatase, receptor type F (PTPRF) removes phosphate group from β-catenin, an event that may subsequently facilitate cell-cell adhesion and ensure the stability of the cadherin complex. This phosphatase has also been implicated in various cellular processes such as neurite growth, nerve regeneration, actin remodeling and regulation of insulin function. Insulin receptor, epidermal growth factor receptor (EGF) receptor, hepatocyte growth factor (HGF) receptor and rearranged during transfection (RET) have been reported as substrates of PTPRF.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.44 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~150 kDa antigen ~210 kDa antigen ~85 kDa |
| species reactivity | human |
| technique(s) | immunoprecipitation (IP): 5-10 μL using lysates of HEK-293T cells over-expressing human PTPRF. western blot: 1:500-1:1000 using lysates of HEK-293T cells over-expressing human PTPRF. |
| UniProt登记号 | P10586 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... PTPRF(5792) mouse ... Ptprf(19268) rat ... Ptprf(360406) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
