Anti-IRF7 Antibody

产品说明

一般描述

Interferon regulatory factor 7 (IRF7) is a member of the IRF family and has 1 IRF tryptophan pentad repeat DNA-binding domain. IRF7 is key transcriptional regulator of type I interferon (IFN)-dependent immune responses and regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. IRF7 plays an important role in the innate immune response against DNA and RNA viruses and has been shown to activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. IRF7 exists in an inactive form in the cytoplasm of uninfected cells and following viral infection it becomes phosphorylated by IKBKE and TBK1 kinases. This leads to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Studies show that IRF7 induces PSMB9/LMP2 expression, either directly or through induction of IRF1 and may play a role in regulating adaptive immune responses. Other evidence shows that IRF7 binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Additionally, IRF7 is implied to activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. IRF7 is expressed predominantly in spleen, thymus and peripheral blood leukocytes.

特异性

This antibody recognizes the C-terminus of IRF7.

免疫原

KLH-conjugated linear peptide corresponding to the C-terminus of human IRF7.
Epitope: C-terminus

应用

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected IRF7 in mouse spleen tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected IRF7 in mouse spleen cells.
This Anti-IRF7 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of IRF7.
Research Sub Category
Immunological Signaling
Research Category
Inflammation & Immunology

质量

Evaluated by Western Blotting in HEK293 whole cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IRF7 in HEK293 whole cell lysate.

目标描述

~51 kDa observed. Uncharacterized bands may appear in some lysate(s).

外形

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Antigen Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HEK293 whole cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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