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PhosphoDetect Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb

品牌
Sigma-Aldrich
货号
553009
规格纯度
liquid, Calbiochem®
参考价格
7533.38 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Recognizes c-Raf phosphorylated at Tyr340 and Tyr341 in HEK293 cells overexpressing c-Raf.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr340/341.
This PhosphoDetect Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb is validated for use in WB, ICC for the detection of c-Raf (pTyr³⁴⁰, Tyr³⁴¹).
Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

免疫原

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
Human

包装

10 tests in Plastic ampoule

应用

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

警告

Toxicity: Standard Handling (A)

外形

In phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
HEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.

其他说明

The immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
Yan, F., and Polk, D.B. 2001. Cancer Res.61, 963.
Yip-Schneider, M.T., et al. 2000. Biochem. J.351, 151.
Mason, C.S. et al. 1999. EMBO J.18, 2137.
Bonner, T.I. et al. 1985. Mol. Cell. Biol.5, 1400.
Moelling, K. et al. 1984. Nature312, 558.
Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA80, 4218.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

产品性质

生物来源rabbit
质量水平100
抗体形式affinity isolated antibody
antibody product typeprimary antibodies
克隆polyclonal
形式liquid
包含≤0.1% sodium azide as preservative
species reactivityrat, mouse, human
manufacturer/tradenameCalbiochem®
储存条件OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型IgG
运输wet ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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