产品说明
一般描述
Monoclonal Anti-AGO1 (rat IgG2a isotype) is derived from the hybridoma 4B8 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a recombinant human AGO1 fusion protein. The Argonaute family of proteins can be subdivided into the Ago subfamily and the Piwi subfamily. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. In human, the Ago subfamily consists of hsAgo1−4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1 is also known to be associated with Golgi and with endoplasmic reticulum. The gene is located on chromosome 1. Eukaryotic initiation factor (EIF2C1)/hAgo1 is expressed in low to medium levels in most tissues, but its expression is particularly high in embryonic kidney and lung.
免疫原
recombinant human AGO1 fusion protein.
应用
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
- Monoclonal Anti-AGO1 antibody produced in rat has been used in:
- western blotting
- immunoprecipitation
- chromatin immunoprecipitation(CHIP) assay
- RNA immunoprecipitation
- RNA-nChIP experiments
生化/生理作用
The Argonaute proteins are evolutionarily conserved between species and have been implicated in both transcriptional and post-transcriptional gene silencing. Eukaryotic initiation factor (EIF2C1)/argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene WT1.The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | rat |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | 4B8, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~95 kDa |
| species reactivity | mouse, human, canine, bovine, monkey |
| 浓度 | ~1 mg/mL |
| technique(s) | immunoprecipitation (IP) western blot: 1-2 μg/mL using HEK-293T cell extracts |
| 同位素/亚型 | IgG2a |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... EIF2C1(26523) mouse ... Eif2c1(236511) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
