Anti-Transcription Initiation Factor IIA subunit 1

产品说明

一般描述

Transcription initiation factor IIA subunit 1 (UniProt: P52655; also known as General transcription factor IIA subunit 1, TFIIAL, Transcription initiation factor TFIIA 42 kDa subunit, TFIIA-42) is encoded by the GTF2A1 (also known as TF2A1) gene (Gene ID: 2957) in human. TFIIA is one of the several components of the transcription machinery of RNA polymerase II that plays an important role in transcriptional activation. TFIIA in a complex with TATA-box-binding protein (TBP) mediates transcriptional activity. After initiation, some components of the pre-initiation complex, including TFIIA, remain attached and re-initiate a subsequent round of transcription. Binding of TFIIA to TBP helps to stabilize TBP binding to the TATA element. TFIIA is a heterodimer of the large unprocessed subunit 1 and a small subunit gamma. It was originally believed to be a heterotrimer of an alpha (p35), a beta (p19) and a gamma subunit (p12).

特异性

This rabbit polyclonal antibody detects Transcription initiation factor IIA subunit 1 in human cells. It targets an epitope within 15 amino acids from the C-terminal region.

免疫原

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Transcription initiation factor IIA subunit 1.

应用

Anti-Transcription Initiation Factor IIA subunit 1, Cat. No. ABE1797, is a highly specific rabbit polyclonal antibody that targets Transcription initiation factor IIA subunit 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Transcription initiation factor IIA subunit 1 in 10 µg of RAW264.7 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Transcription initiation factor IIA subunit 1 in human brain and human testis tissue.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Transcription initiation factor IIA subunit 1 in 10 µg of HeLa cell lysate.

目标描述

~55 kDa observed; 41.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

产品性质