产品说明
一般描述
Monoclonal Anti-Melan-A (mouse IgG1 isotype) is derived from the hybridoma A103 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with a human recombinant Melan-A protein. Melan-A (also known as MART1) is a melanoma antigen recognized by T cells. The protein is expressed mainly in cells and tumors of melanocytic lineage and thus belongs to the melanocyte differentiation markers.
Melan-A is also known as MART-1 (melanoma antigen recognized by T-cells 1). It is a transmembrane protein which is hydrophobic in nature. Melan-A is expressed in melanocytes and melanoma tumors.
免疫原
recombinant human Melan-A protein.
应用
Anti-Melan-A antibody, Mouse monoclonal has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
- immunoprecipitation
- immunohistochemistry
生化/生理作用
Melan-A/MART1 may have a role in pigmentation since its expression pattern and sub cellular distribution is similar to other melanosomal proteins and correlates with melanin content. Melan-A is important for Pmel17 activity, forms a complex with it, and affects its expression, stability, trafficking, and processing that is required for melanosome structure and maturation. Thus, Melan-A is important for Pmel17 function and therefore it is important for mammalian pigmentation.
目标描述
Melan-A is a melanoma antigen recognized by T cells.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD01633630 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | A103, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~22 kDa |
| species reactivity | canine, human |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry immunohistochemistry immunoprecipitation (IP) indirect ELISA western blot: 0.1-0.2 μg/mL using total cell extract of SKMEL19 cells ( |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | Q16655 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... MLANA(2315) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
