Anti-MafK/Nfe2u Antibody

产品说明

一般描述

Transcription factor MafK (UniProt Q61827; also known as Erythroid transcription factor NF-E2 p18 subunit, Nuclear factor erythroid derived 2 ubiquitous, p18 NF-E2) is encoded by the Mafk (also known as Nfe2u, AW061068) gene (Gene ID 17135) in murine species. MafK, MafG and MafF consistute a family of transcription factors that dimerize with the CNC (cap′ n′ collar) family regulatory protein Nrf2 (NF-E2 p45-related factor 2) via their bZip domain. The Nrf2/small Maf heterodimer regulates downstream genes, including those of phase II detoxifying enzymes, by targeting antioxidant responsive element (ARE) and Maf recognition element (MARE).

免疫原

Maltose binding protein-tagged recombinant protein corresponding to mouse MafK/Nfe2u.

应用

This Anti-MafK/Nfe2u Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of MafK/Nfe2u.
Research Sub Category
Nuclear Receptors
Western Blotting Analysis: A representative lot detected endogenous MafK as well as Zn+2-induced expression of exogenous MafK using nuclear extracts from a mouse erythroleukemia (MEL) cell line transfected with MafK cDNA in a metallothionein IIA gene promoter-driven expression plasmid (Igarashi, K., et al. (1995). Proc Natl Acad Sci U S A. 92(16):7445-7449).
Chromatin Immunoprecipitation Analysis: A representative lot detected MafK association with the GST-P enhancer (GPE1) in rat hepatoma cells (H4IIE and dRLh84) and hyperplastic nodules/HN-bearing pre-neoplastic rat hepatocytes, but not in normal rat hepatocytes. While MafK association with the NAD(P)H:quinone oxidoreductase 1 (NQO1) antioxidant responsive element (ARE) was detected in all four sample types (Ikeda, H., et al. (2004). Biochem J. 380(Pt 2):515-521).
Electrophoretic Mobility Shift Assay Analysis: A representative lot caused a supershift of MafK-containing complex in EMSA using radiolabeled oligonucleotides containing PBGD gene NF-E2 binding site and nuclear extracts from a mouse erythroleukemia (MEL) cell line induced to overexpress MafK (Igarashi, K., et al. (1995). Proc Natl Acad Sci U S A. 92(16):7445-7449).
Electrophoretic Mobility Shift Assay (EMSA): A representative lot caused a supershift of both the constitutive and tBHQ-induced ARE-binding complex (Complex I and II, respectively) in EMSA using a radiolabeled oligonucleotide containing antioxidant responsive element (ARE) NF-E2 binding site and nuclear extracts from untreated and tBHQ-treated K562 cells (Kim, Y.C., et al. (2003). Oncogene. 22(12):1860-1865).
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in MEL (mouse erythroleukemia) cell lysate.

Western Blotting Analysis: 1:2,000 dilution of this antibody detected MafK/NF-E2 in 20 µg of MEL (mouse erythroleukemia) cell lysate.

目标描述

~18 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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