产品说明
一般描述
Nestin (NES) is a class VI cytoplasmic protein which is expressed in the intermediate filaments. It has a molecular weight of 177.4kDa. The gene encoding it is localized on human chromosome 1q23.1 and has four exons.
Nestin is expressed during early stages of development in the central nervous system (CNS) and in myogenesis. Nestin is found in neurogenic progenitor cells (NPCs) and has been used as a marker for neural progenitor cells.
免疫原
synthetic peptide corresponding to a sequence at the C-terminal region of human nestin, conjugated to KLH. The sequence is highly conserved (72% identity) in rat and mouse nestin.
应用
Anti-Nestin (C-terminal region) antibody produced in rabbit has been used in western blotting and immunostaining.
Anti-Nestin (C-terminal region) antibody has been used in
- immunofluorescence
- Proximity ligation assay
- immunohistofluorescence staining
- immunofluorescence labeling
- immunoblotting
生化/生理作用
Nestin (NES) functions as a modulator of proteins which are involved in cell growth. It also interacts with other proteins of the intermediate filament. NES has been studied as a marker of neural stem cells.
Nestin expression is down-regulated and replaced by the expression of other intermediate filament proteins such as neurofilament and Glial fibrillary acidic protein (GFAP). Nestin expression is re-induced in the adult organism during pathological conditions, such as formation of glial scar after injury to the central nervous system (CNS) and in certain types of CNS tumors. Nestin has been shown to play an important role in the phosphorylation-dependent disassembly of vimentin intermediate filaments during mitosis and is a potential target for Cyclin-dependent kinase 5 (cdk5) and p35 during development.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| NACRES | NA.41 |
产品性质
| 生物来源 | rabbit |
| 质量水平 | 200 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~300 kDa |
| species reactivity | mouse, human, rat |
| 浓度 | ~1.5 mg/mL |
| technique(s) | indirect immunofluorescence: 1-2 μg/mL using mouse P19 cells. western blot: 2-4 μg/mL using lysates of HEK293T cells over-expressing rat nestin. |
| UniProt登记号 | P48681 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... NES(10763) mouse ... Nes(18008) rat ... Nes(25491) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
