产品说明
一般描述
Anti-Cdc7 Kinase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the DCS-341 hybridoma produced by the fusion of mouse myeloma cells (NS2) and splenocytes from BALB/c mice immunized with full length human Cdc7 kinase. Cell division cycle 7-related protein kinase(CDC7) is an enzyme encoded by the CDC7 gene in humans. It is highly expressed in diffuse large B cell lymphoma (DLBCL). Human Cdc7 kinase consists of 574 amino acids with a molecular weight of 55 kDa. CDC7 shares nearly 45% identity with yeast Cdc7 kinase in the kinase domain.
免疫原
full-length human Cdc7 kinase.
应用
Anti-Cdc7 Kinase antibody, Mouse monoclonal has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry.
包装
200 μL in glass insert
生化/生理作用
Cell division cycle 7-related protein kinase (CDC7) in addition to its role in chromosome structure maintenance and repair, is also involved in meiosis and checkpoint responses.
Cell division cycle 7-related protein kinase(CDC7) is a serine-threonine essential kinase required for the initiation of DNA replication and is involved in regulation of the cell cycle. It is high expressed in diffuse large B cell lymphoma (DLBCL). Loss of Cdc7 kinase expression results in retarded growth at both cellular and whole body levels, with especially profound impairment of germ cell development. It inhibits genotoxin-induced apoptosis and increases survival in cancer cells. It may play an important role in the development and progression of colorectal cancer. Depletion of Cdc7 induces cancer cell death regardless of its p53 status. It can also interact with and phosphorylate Claspin.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD04975117 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | DCS-341, monoclonal |
| 形式 | buffered aqueous solution |
| species reactivity | human |
| 浓度 | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable microarray: suitable western blot: 0.4 μg/mL using total cell extract of A431 cells |
| 同位素/亚型 | IgG1 |
| UniProt登记号 | O00311 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... CDC7(8317) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
