产品说明
一般描述
Monoclonal Anti-Importin α (mouse IgG2b isotype) is derived from the hybridoma IM-75 produced by the fusion of mouse myeloma cells (NS1 cells), and splenocytes from BALB/c mice, immunized with recombinant human importin α. The Importin α family of proteins is comprised of three subfamilies based on amino acid sequence similarity: SRP1-like subfamily (containing the SRP1, importin α5, α6 and α7), Rch1-like subfamily (containing Rch1, pendulin, importin α2), and α3/Qip1-like subfamily (containing α4).
免疫原
recombinant human importin α.
应用
Anti-Importin α antibody, Mouse monoclonal has been used:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
Monoclonal Anti-Importin α antibody produced in mouse has been used in western blotting.
生化/生理作用
The gene KPNA4 (karyopherin subunit α 4) encodes a 16kDa protein, also referred to as importin α 3 belonging to the family of karyopherins (designated KPNA1 to KPNA4). It participates in the recognition of NLS (nuclear localization sequence) sites and dock NLS-containing proteins destined for transport into the nuclear pore complex. It binds to translocon and participates in the sorting of INM (inner nuclear membrane)-directed integral membrane proteins. It associates with nucleoporins and functions in cargo-translocation across the nuclear membrane. Each KPNA contains an N-terminal repeat domain called the importin β binding site that binds to KPNB1, which in turn directs each KPNA/cargo complex through the nuclear pore. The encoded shuttling protein also functions in NF (nuclear factor)-κB nuclear translocation.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD06798706 |
| NACRES | NA.41 |
产品性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | unconjugated |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | IM-75, monoclonal |
| 形式 | buffered aqueous solution |
| 分子量 | antigen ~60 kDa |
| species reactivity | bovine, human, mouse, rat |
| technique(s) | immunocytochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable western blot: 1-2 μg/mL using HeLa cell extract |
| 同位素/亚型 | IgG2b |
| UniProt登记号 | O00629 |
| 运输 | dry ice |
| 储存温度 | −20℃ |
| Gene Information | human ... KPNA4(3840) mouse ... Kpna4(16649) rat ... Kpna4(361959) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
