产品说明
一般描述
PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.
应用
Pronase® Protease, Streptomyces griseus, nuclease-free has been used:
- in single-cell preparations for cardiac side population (CSP) analysis
- in aflatoxin plasma biomarker assay
- to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion
包装
10, 100, 5, 50 ku in Plastic ampoule
25 ku in Glass bottle
生化/生理作用
Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.
警告
Toxicity: Harmful (C)
单位定义
One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.
重悬
Unstable in solution; reconstitute just prior to use.
其他说明
Note: 1 KU = 1000 units.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| MDL编号 | MFCD00132092 |
产品性质
| 形式 | lyophilized |
| specific activity | ≥70,000 proteolytic units/g dry wt |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 溶解性 | water: 10 mg/mL |
| 异质活性 | Nuclease, none detected |
| 运输 | ambient |
| 储存温度 | 2-8℃ |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H334 - H335 |
| 预防措施声明 | P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 2 |
