产品说明
一般描述
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
应用
Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.
包装
1 g in glass bottle
100, 250 mg in glass bottle
生化/生理作用
Lys-acetylated trypsin is less susceptible to autolysis than the native form.
单位定义
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).
制备说明
Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).
其他说明
View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.
基本信息
| 酶学委员会(EC)编号 | 3.4.21.4 ( BRENDA | IUBMB ) |
| MDL编号 | MFCD00082094 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 类型 | Type V-S |
| 形式 | powder |
| specific activity | ≥8,500 BAEE units/mg protein (biuret) |
| UniProt登记号 | Q29463 |
| 储存温度 | −20℃ |
| Gene Information | cow ... TRYP8(282603) |
安全信息
| 象形图 |   |
| 警示用语： | Danger |
| 危险声明 | H315 - H319 - H334 - H335 |
| 预防措施声明 | P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
