产品说明
一般描述
JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
应用
JumpStart™ REDTaq® DNA Polymerase has been used in:
- polymerase chain reaction (PCR) for the amplification of insulin-enterotoxin ricin fusion gene (INS-RTB)
- endothelial cells DNA derived from reverse transcribed RNA
- leg genomic DNA from cricket flies
- for DNA methylation analysis
- for the amplification of mitochondrial gene by conventional PCR
特点和优势
- Reduces non-specific amplification
- Increased target yield and specificity
- Higher the amplification irrespective of the target concentration
- Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
- Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
- Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
- Assembled PCR reactions can be placed at room temperature for up to 2 hours
包装
The enzyme is provided with an optimized 10× reaction buffer.
单位定义
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 ℃.
其他说明
View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.
法律信息
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of sigma-aldrich Co. LLC
REDTaq is a registered trademark of sigma-aldrich Co. LLC
基本信息
| MDL编号 | MFCD00166026 |
| NACRES | NA.55 |
产品性质
| 质量水平 | 200 |
| 形式 | liquid |
| 用途 | sufficient for 250 reactions sufficient for 2500 reactions sufficient for 50 reactions |
| 特点 | dNTPs included: no hotstart |
| 浓度 | 1 unit/μL |
| technique(s) | PCR: suitable |
| 颜色 | red |
| input | purified DNA |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H315 - H319 - H335 |
| 预防措施声明 | P302 + P352 - P305 + P351 + P338 |
| 危险分类 | Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type ABEK (EN14387) respirator filter |
