FLYA13

产品说明

细胞系来源

Human fibrosarcoma (retroviral packaging cells)

细胞系描述

FLYA13 has been developed together with FLYRD18 (Sigma catalog number 95091902) for packaging of retroviruses, it is especially useful for in vivo gene therapy. Both cell lines have been derived from HT1080 cells (Sigma catalog number 85111505). FLYA13 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of amphotropic murine leukaemia virus. This cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000 i.u./ml. Addition of 4μg/ml blasticidin S and 10μg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y,
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

培养基

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:20, i.e., seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 ℃.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

产品性质