产品说明
细胞系来源
Human Caucasian promyelocytic leukaemia
细胞系描述
Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.
应用
Differentiation studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃. Do not allow the culture medium to become acidic or the cell line will differentiate.
其他说明
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产品性质
| 生物来源 | human blood |
| 生长模式 | Suspension |
| 核型 | Modal no. = 93, Hypertetraploid |
| 形态学 | Lymphoblastoid |
| 产品 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
