Anti-Cbl Antibody, clone 7G10

产品说明

一般描述

Casitas B-lineage lymphoma (Cbl), is an oncogene which causes pre B-cell lymphomas. It participates in the regulation of cell signaling and has the ability to interact with many proteins such as receptor/non-receptor PTK′s, structural proteins, and novel proteins that are not well understood. Diseases associated with Cbl are autoimmune diseases, diabetes, and leukemia. By modifying Cbl′s ability to bind to oncogenic proteins, Cbl may be developed into an anti-cancer tool and in the near future used in therapeutic intervention.

特异性

Antibody recognizes amino acids 878-906 on human Cbl

免疫原

Epitope: a.a. 878-906
GST fusion protein corresponding to residues 878-906 of human Cbl

应用

This Anti-Cbl Antibody, clone 7G10 is validated for use in IP, WB, IC for the detection of Cbl.
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Immunocytochemistry:
5-10 μg/mL of a previous lot showed positive immunostaining for Cbl in 3T3/A31 cells fixed with ice cold Methanol/Ethanol [1:1] for 1 minute.
Immunoprecipitation:
2 μg of a previous lot immunoprecipitated Cbl from 1 mg of a mouse 3T3/A31 cell RIPA lysate, as reported by an independent laboratory.
Research Category
Protein Trafficking

质量

routinely evaluated by immunoblot on RIPA lysates from human Jurkat, mouse 3T3/A31, TF-1, or FDC-P1/Mac11

目标描述

120kDa

外形

Protein G Chromatography
Purified IgG1k in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4
Format: Purified

储存及稳定性

Stable for 1 year at 2-8ºC from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

分析说明

Control
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μl of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20μg of reduced lysate per lane for minigels

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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