Anti-Tie2/TEK Antibody, clone Ab33

产品说明

一般描述

TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

特异性

Recognizes Tie2/TEK, Mr 145kDa

免疫原

Fusion protein corresponding to residues 1-745 of human Tie2/TEK with a C-terminal His6-tag purified from infected SF-9 cells. Clone Ab33

应用

This Anti-Tie2/TEK Antibody, clone Ab33 is validated for use in WB, IP, IH for the detection of Tie2/TEK.
Immunoprecipitation: This antibody immunoprecipitated Tie2/TEK, as reported by an independent laboratory.
Immunohistochemistry: Reported to detect Tie2/TEK in frozen, Triton-treated sections.
This antibody is not suitable for paraffin-embedded sections.

质量

Evaluated by western blot on RIPA lysates from HUVEC cells.
Western Blot Analysis: 0.2-1 μg/mL of this antibody detected Tie2/TEK in RIPA lysates from HUVEC cells.

目标描述

145 kDa

外形

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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