产品说明
一般描述
A cell-permeable anacardic acid (Cat. No. 172050) analog that inhibits protein SUMO (Cat. Nos. 662037, 662039, and 662042) modification (IC50 = 3.0 µM using RanGAP1-C2 as substrate) in an ATP-dependent manner by selectively targeting SUMO-activating enzyme E1 (Cat. Nos. 662073 and 662074) and interfering with E1-SUMO intermediate formation independent of E1 active site cysteine. Both ginkgolic acid and anacardic acid are shown to effectively decrease overall SUMOylation of 293T cellular proteins, including p53, in a dose-dependent manner, while neither compound is effective in affecting overall cellular protein ubiquitination or histone H4K8 acetylation in 293T cultures, although both compounds are shown to inhibit histone lysine acetylation by PCAF (Cat. No. 124026) in cell-free acetylase assays. Also available as a 25 mM solution in DMSO (Cat. No. 508197).
A cell-permeable anacardic acid (Cat. No. 172050) analog that inhibits protein SUMO (Cat. No. 662037, 662039, and 662042) modification (IC50 = 3.0 µM using RanGAP1-C2 as substrate) in an ATP-dependent manner by selectively targeting SUMO-activating enzyme E1 (Cat. No. 662073 and 662074) and interfering with E1-SUMO intermediate formation independent of E1 active site cysteine. Both ginkgolic acid and anacardic acid are shown to effectively decrease overall SUMOylation of 293T cellular proteins, including p53, in a dose-dependent manner, while neither compound is effective in affecting overall cellular protein ubiquitination or histone H4K8 acetylation in 293T cultures, although both compounds are shown to inhibit histone lysine acetylation by PCAF (Cat. No. 124026) in cell-free acetylase assays.
包装
5 mg in Plastic ampoule
Packaged under inert gas
警告
Toxicity: Irritant (B)
重悬
Following intitial thaw, aliquot and freeze (-20°C).
其他说明
Fukuda, I., et al. 2009. Chem. Biol.16, 133.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
经验(实验)分子式 | C22H34O3 |
分子量 | 346.50 |
MDL编号 | MFCD03093717 |
产品性质
质量水平 | 100 |
测定 | ≥98% (HPLC) |
形式 | solid |
manufacturer/tradename | Calbiochem® |
储存条件 | OK to freeze protect from light |
颜色 | white |
溶解性 | DMSO: 100 mg/mL ethanol: 100 mg/mL |
运输 | ambient |
储存温度 | −20℃ |
InChI | 1S/C22H34O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-16-19-17-15-18-20(23)21(19)22(24)25/h7-8,15,17-18,23H,2-6,9-14,16H2,1H3,(H,24,25)/b8-7- |
InChI key | YXHVCZZLWZYHSA-FPLPWBNLSA-N |
安全信息
象形图 | |
警示用语: | Warning |
危险声明 | H317 - H413 |
预防措施声明 | P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313 |
危险分类 | Aquatic Chronic 4 - Skin Sens. 1 |
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 3 |
闪点(F) | 509.9 °F - (calculated) |
闪点(C) | 265.5 ℃ - (calculated) |