产品说明
一般描述
Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Highly purified native enzyme derived from cell culture. Secreted by several cell types. Exists in a stable, but non-covalent 1:1 complex with TIMP-2. Requires activation prior to use.
Note: 1 mU = 1 milliunit.
Native pro-MMP-2 from cultured, human rheumatoid synovial fibroblasts. Secreted by several cell types. Requires activation just prior to use.
包装
5 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.
外形
In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.
制备说明
Prepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
重悬
Following initial thaw, aliquot and freeze (-70°C).
其他说明
Buisson-Leqendre, N., et al. 2004. Arth. Rheumat.50, 2151.
Morgunova, E., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7414.
Kolkenbrock, H., et al. 1997. Biol. Chem. 378, 71.
法律信息
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 酶学委员会(EC)编号 | 3.4.24.24 ( BRENDA | IUBMB ) |
产品性质
| 测定 | ≥90% (SDS-PAGE) |
| 质量水平 | 100 |
| 形式 | liquid |
| specific activity | ≥600 mU/mg protein |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 溶解性 | aqueous buffer: soluble |
| 异质活性 | other MMP activity, none detected |
| 运输 | wet ice |
| 储存温度 | −70℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
