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Anti-LRP1 85kDa subunit Antibody, clone 4G7, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1311
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2138.93 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 4G7 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects the 85 kDa subunit of LRP-1. It targets an epitope within the C-terminal cytoplasmic domain.

免疫原

His-tagged recombinant fragment corresponding to 100 amino acids from the C-terminal, cytoplasmic domain of human Prolow-density lipoprotein receptor-related protein 1 (LRP-1).

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRP1 85 kDa subunit in HEK293 cell lysate.
Flow Cytometry Analysis: 1 μg from a representative lot detected LRP1 85kDa subunit in one million HEK293 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LRP1 85kDa subunit in C6, PC12, C2C12, SH-SY5Y cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-LRP1 85kDa subunit, clone 4G7 ZooMAb, Cat. No. ZRB1311, is a recombinant rabbit monoclonal antibody that specifically detects LRP1 85kDa subunit and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.

目标描述

Prolow-density lipoprotein receptor-related protein 1 (UniProt Q07954; also known as A2MR, Alpha-2-macroglobulin receptor, APER, Apolipoprotein E receptor, CD19. LRP-1) is encoded by the LRP1 (also known as A2MR, APR) gene (Gene ID: 4035) in human. LRP-1 is single pass type I membrane protein that is expressed in most tissues, but is abundant in liver, brain, and lung. It serves as a major VSMC receptor with a crucial role in maintaining vessel wall integrity and its expression in adult smooth muscle cells is shown to be essential for maintaining a contractile phenotype. LRP1 is synthesized with a signal peptide sequence (aa 1-19) and is processed in trans-Golgi network by Furin to generate a 515 kDa alpha subunit and an 85 kDa beta subunit that contains the 100-amino intracellular domain (LRPICD; aa 4445-4544). The alpha and beta subunits are non-covalently linked during LRP1 transport to the cell membrane. LRP1 recognizes and mediates the endocytosis of more than 40 different ligands, including apolipoprotein E (ApoE), APP and amyloid beta. It is the primary receptor mediating transport of amyloid beta peptides across the blood-brain barrier into circulation, thereby clearing them from the brain. LRP1 is required for early embryonic development and is involved in cellular lipid homeostasis and plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin). Excessive copper accumulation in the brain has been linked with reduced LRP1 mediated clearance of amyloid beta peptides across the blood brain barrier, which may contribute to complications of Alzheimer′s disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Au, DT et al. (2018). Arterioscler. Thromb. Vasc. Biol. 38(11); 2651- 2664).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆4G7, monoclonal
recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
species reactivityhuman, mouse, rat
包装pkg of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

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