Anti-STAT1 (isoform alpha) Antibody, clone 1D4 ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1D4 is a a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human STAT1. It targets an epitope within 38 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 38 amino acids from the C-terminal region of human Signal transducer and activator of transcription 1-alpha/beta (STAT1) protein.

应用

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected STAT1 in HEK293 cell lysate.
Immunocytochemistry Analysis: A 1:100-1,000 dilution from a representative lot detected STAT1 in U2OS and Hela cells treated with interferon-gamma.
Immunohistochemistry (Paraffin) Analysis: A 1:100-1,000 dilution from a representative lot detected STAT1 in human kidney and human breast cancer tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound recombinant STAT1 alpha fragment with a KD of 1.1 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Rabbit recombinant monoclonal antibody Anti-STAT1 (isoform ), clone 1D4 ZooMAb Rabbit Monoclonal, Cat. No. ZRB1459 is used in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry and Affinity Binding Assay, for the detection of STAT1.

目标描述

Signal transducer and activator of transcription 1-alpha/beta (UniProt: P42224; also known as Transcription factor ISGF-3 components p91/p84, STAT1) is encoded by the STAT1 gene (Gene ID: 6772) in human. STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling proteins. STAT1 is activated by many different ligands including interferons, interleukins, and growth factors. STAT1 exists in two forms, the 91 kDa STAT1alpha and the 84 kDa STAT1beta, which are encoded by the same gene with splicing variant. Following ligand binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases and tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and enter the nucleus. Phosphorylation on serine 727 by several kinases including MAPK14, ERK1/2 and CAMKII following on IFN-gamma stimulation, regulates STAT1 transcriptional activity. This phosphorylation also promotes sumoylation though increasing interaction with PIAS. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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