Anti-PLA2R1 Antibody, clone 1D5, ZooMAb^® Rabbit Monoclonal

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1D5 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Secretory phospholipase A2 receptor (PLA2R1). It targets an epitope with in the extracellular domain.

免疫原

GST/His-tagged recombinant fragment corresponding to 136 amino acids from the N-terminal, extracellular domain region of human Secretory phospholipase A2 receptor (PLA2R1).

应用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected PLA2R1 in HeLa, A431, HUVEC and NIH 3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PLA2R1 in human kidney tissue sections.
Anti-PLA2R1, clone 1D5, ZooMAb, Cat. No. ZRB012657, is a recombinant Rabbit Monoclonal antibody that detects Secretory phospholipase A2 receptor and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Secretory phospholipase A2 receptor (UniProt: also known as PLA2-R, PLA2R, 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13-member C, M-type receptor) is encoded by the PLA2R1 (also known as CLEC13C) gene (Gene ID: 22925) in human. PLA2R is a single-pass type I membrane protein that serves as a receptor for secretory phospholipase A2 (sPLA2). It is highly expressed in kidney and is also present in lung macrophages, pancreas, amnion, and placenta. PLA2R is a receptor for phospholipase sPLA2-IB/PLA2G1B, but not sPLA2-IIA/PLA2G2A. It can also bind to snake PA2-like toxins. Binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions and clearance. Binding of sPLA2-IB/PLA2G1B is shown to induce various effects depending on the cell type, such as activation of the mitogen-activated protein kinase cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. PLA2R is also involved in responses in proinflammatory cytokine productions during endotoxic shock. The circulating, soluble form of secretory phospholipase A2 receptor acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. PLA2R is initially produced with a signal peptide sequence (aa 1-20), the cleavage of which produced the mature form that has a large extracellular domain (aa 21-1397), a transmembrane domain (aa 1398-1418), and a cytoplasmic tail (aa 1419-1463). The extracellular domain contains a fibronectin-like type II domain (173-221), and a tandem repeat of eight carbohydrate-recognition domains (C-type lectins). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

120 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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