位置:首页 > 品牌 > Sigma-Aldrich >

Anti-Bax Antibody, clone 1C7, ZooMAb® Rabbit Monoclonal

品牌
Sigma-Aldrich
货号
ZRB1103
规格纯度
recombinant, expressed in HEK 293 cells
参考价格
2001.06 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 1C7 ZooMAb is a rabbit recombinant monoclonal antibody that specifically detects human Apoptosis regulator BAX. It targets an epitope within 20 amino acids from the N-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Apoptosis regulator BAX.

应用

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Bax in MCF7 cells treated with Etoposide (50 uM 6 h).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bax in human kidney and human lung cancer tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Bax in human kidney tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Anti-Bax, clone 1C7 ZooMAb, Cat. No. ZRB1103, is a recombinant rabbit monoclonal antibody that specifically targets Bax and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

目标描述

Apoptosis regulator BAX (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4, Bax) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is single-pass membrane protein located on the mitochondrion outer membrane. It is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Under normal conditions, Bax is largely cytosolic via constant retro-translocation from mitochondria to the cytosol that is helpful in avoiding its accumulation at the mitochondrial outer membrane. Upon induction of apoptosis, Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. Bax is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Bax contains 3 BH motifs (BH3: aa 59-73; BH1: aa 98-118; BH2: aa 150-165) and intact BH3 motif is shown to be required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. Eight different isoforms of Bax have been described that are produced by alternative splicing. It is pressed in a wide variety of tissues. Isoform alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Psi is found in glial tumors. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8℃; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8℃, or -20℃ for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品性质

生物来源rabbit (recombinant)
重组expressed in HEK 293 cells
偶联物unconjugated
抗体形式purified antibody
antibody product typeprimary antibodies
克隆1C7, recombinant monoclonal
产品线ZooMAb® learn more
形式lyophilized
分子量calculated mol wt 21.18 kDa
observed mol wt ~21 kDa
species reactivityhuman
包装antibody small pack of 25 μL
环保替代产品特性Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
增强验证recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)
同位素/亚型IgG
环保替代产品分类Aligned
运输ambient
储存温度2-8℃

安全信息

储存分类代码11 - Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

推荐产品
| 首页 | 联系我们 | 会员服务 | 广告服务 | 友情链接 |
版权所有 CopyRight © 2008-2024 粤ICP备08119708号